cd47 neutralizing mab Search Results


anti cd47 neutralizing antibody  (Bio X Cell)
95
Bio X Cell anti cd47 neutralizing antibody
a – f MS1 endothelial cells ( a , c , e ) or primary HUVECs ( b , d , f ) were infected with lentiviruses encoding a shRNA directed against IRE1α ( shErn1 or shERN1 ) or a scramble control ( shCtrl ) for 48 hours. a , b Quantitative RT-PCR analysis of Xbp1 mRNA splicing and Thbs1/THBS1 mRNA abundance in MS1 cells ( a ) or HUVECs ( b ) pre-cultured for 4 hours at 5 mM glucose or following stimulation for 24 hours with high glucose at 16 mM ( n = 3 independent experiments). c , d Immunoblot analysis of IRE1α and TSP1 protein levels in MS1 cell lysates ( c ) or HUVEC lysates ( d ) when cultured at 5 mM versus 16 mM glucose for 24 hours. α-Tubulin was used as a loading control (n = 3 independent experiments). e , f Immunoblot analysis of TSP1 protein in culture medium of MS1 cells ( e ) or HUVECs ( f ). Cell lysate α-Tubulin was used as a loading control ( n = 3 independent experiments). g Cell proliferation and viability analysis by cell-count assay or using the CCK-8 Kit for lentivirus-infected MS1 cells cultured at 16 mM glucose (n = 2 independent experiments). h , i MS1 cells were infected with shCtrl , shErn1 , or both shErn1 and shThbs1 lentiviruses for 48 hours. h Immunoblot analysis of IRE1α and TSP1 proteins in MS1 cell lysates. i MS1 cell proliferation analysis by cell-count assay or using the CCK-8 Kit when cultured at 16 mM glucose ( n = 2 independent experiments). j Proliferation analysis by the CCK-8 Kit of MS1 cells that were cultured at 16 mM glucose and treated with PBS (Vehicle, Veh.) or 1 μg/mL recombinant human TSP1 protein in the absence or presence of 1 μg/mL <t>anti-CD47</t> neutralizing antibody for 48 hours ( n = 6 independent treatment experiments). Data are shown as mean ± SEM by two-way ANOVA ( a – d , g , i , * indicates shCtrl versus shErn1 ; # indicates shErn1 versus shErn1/shThbs1 in i ) or unpaired two-tailed Student’s t -test ( e , f , j ).
Anti Cd47 Neutralizing Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd47 neutralizing antibody/product/Bio X Cell
Average 95 stars, based on 1 article reviews
anti cd47 neutralizing antibody - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
cd47 neutralizing mab  (Bio X Cell)
96
Bio X Cell cd47 neutralizing mab
a – f MS1 endothelial cells ( a , c , e ) or primary HUVECs ( b , d , f ) were infected with lentiviruses encoding a shRNA directed against IRE1α ( shErn1 or shERN1 ) or a scramble control ( shCtrl ) for 48 hours. a , b Quantitative RT-PCR analysis of Xbp1 mRNA splicing and Thbs1/THBS1 mRNA abundance in MS1 cells ( a ) or HUVECs ( b ) pre-cultured for 4 hours at 5 mM glucose or following stimulation for 24 hours with high glucose at 16 mM ( n = 3 independent experiments). c , d Immunoblot analysis of IRE1α and TSP1 protein levels in MS1 cell lysates ( c ) or HUVEC lysates ( d ) when cultured at 5 mM versus 16 mM glucose for 24 hours. α-Tubulin was used as a loading control (n = 3 independent experiments). e , f Immunoblot analysis of TSP1 protein in culture medium of MS1 cells ( e ) or HUVECs ( f ). Cell lysate α-Tubulin was used as a loading control ( n = 3 independent experiments). g Cell proliferation and viability analysis by cell-count assay or using the CCK-8 Kit for lentivirus-infected MS1 cells cultured at 16 mM glucose (n = 2 independent experiments). h , i MS1 cells were infected with shCtrl , shErn1 , or both shErn1 and shThbs1 lentiviruses for 48 hours. h Immunoblot analysis of IRE1α and TSP1 proteins in MS1 cell lysates. i MS1 cell proliferation analysis by cell-count assay or using the CCK-8 Kit when cultured at 16 mM glucose ( n = 2 independent experiments). j Proliferation analysis by the CCK-8 Kit of MS1 cells that were cultured at 16 mM glucose and treated with PBS (Vehicle, Veh.) or 1 μg/mL recombinant human TSP1 protein in the absence or presence of 1 μg/mL <t>anti-CD47</t> neutralizing antibody for 48 hours ( n = 6 independent treatment experiments). Data are shown as mean ± SEM by two-way ANOVA ( a – d , g , i , * indicates shCtrl versus shErn1 ; # indicates shErn1 versus shErn1/shThbs1 in i ) or unpaired two-tailed Student’s t -test ( e , f , j ).
Cd47 Neutralizing Mab, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd47 neutralizing mab/product/Bio X Cell
Average 96 stars, based on 1 article reviews
cd47 neutralizing mab - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier

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a – f MS1 endothelial cells ( a , c , e ) or primary HUVECs ( b , d , f ) were infected with lentiviruses encoding a shRNA directed against IRE1α ( shErn1 or shERN1 ) or a scramble control ( shCtrl ) for 48 hours. a , b Quantitative RT-PCR analysis of Xbp1 mRNA splicing and Thbs1/THBS1 mRNA abundance in MS1 cells ( a ) or HUVECs ( b ) pre-cultured for 4 hours at 5 mM glucose or following stimulation for 24 hours with high glucose at 16 mM ( n = 3 independent experiments). c , d Immunoblot analysis of IRE1α and TSP1 protein levels in MS1 cell lysates ( c ) or HUVEC lysates ( d ) when cultured at 5 mM versus 16 mM glucose for 24 hours. α-Tubulin was used as a loading control (n = 3 independent experiments). e , f Immunoblot analysis of TSP1 protein in culture medium of MS1 cells ( e ) or HUVECs ( f ). Cell lysate α-Tubulin was used as a loading control ( n = 3 independent experiments). g Cell proliferation and viability analysis by cell-count assay or using the CCK-8 Kit for lentivirus-infected MS1 cells cultured at 16 mM glucose (n = 2 independent experiments). h , i MS1 cells were infected with shCtrl , shErn1 , or both shErn1 and shThbs1 lentiviruses for 48 hours. h Immunoblot analysis of IRE1α and TSP1 proteins in MS1 cell lysates. i MS1 cell proliferation analysis by cell-count assay or using the CCK-8 Kit when cultured at 16 mM glucose ( n = 2 independent experiments). j Proliferation analysis by the CCK-8 Kit of MS1 cells that were cultured at 16 mM glucose and treated with PBS (Vehicle, Veh.) or 1 μg/mL recombinant human TSP1 protein in the absence or presence of 1 μg/mL anti-CD47 neutralizing antibody for 48 hours ( n = 6 independent treatment experiments). Data are shown as mean ± SEM by two-way ANOVA ( a – d , g , i , * indicates shCtrl versus shErn1 ; # indicates shErn1 versus shErn1/shThbs1 in i ) or unpaired two-tailed Student’s t -test ( e , f , j ).

Journal: Nature Communications

Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets

doi: 10.1038/s41467-025-68276-1

Figure Lengend Snippet: a – f MS1 endothelial cells ( a , c , e ) or primary HUVECs ( b , d , f ) were infected with lentiviruses encoding a shRNA directed against IRE1α ( shErn1 or shERN1 ) or a scramble control ( shCtrl ) for 48 hours. a , b Quantitative RT-PCR analysis of Xbp1 mRNA splicing and Thbs1/THBS1 mRNA abundance in MS1 cells ( a ) or HUVECs ( b ) pre-cultured for 4 hours at 5 mM glucose or following stimulation for 24 hours with high glucose at 16 mM ( n = 3 independent experiments). c , d Immunoblot analysis of IRE1α and TSP1 protein levels in MS1 cell lysates ( c ) or HUVEC lysates ( d ) when cultured at 5 mM versus 16 mM glucose for 24 hours. α-Tubulin was used as a loading control (n = 3 independent experiments). e , f Immunoblot analysis of TSP1 protein in culture medium of MS1 cells ( e ) or HUVECs ( f ). Cell lysate α-Tubulin was used as a loading control ( n = 3 independent experiments). g Cell proliferation and viability analysis by cell-count assay or using the CCK-8 Kit for lentivirus-infected MS1 cells cultured at 16 mM glucose (n = 2 independent experiments). h , i MS1 cells were infected with shCtrl , shErn1 , or both shErn1 and shThbs1 lentiviruses for 48 hours. h Immunoblot analysis of IRE1α and TSP1 proteins in MS1 cell lysates. i MS1 cell proliferation analysis by cell-count assay or using the CCK-8 Kit when cultured at 16 mM glucose ( n = 2 independent experiments). j Proliferation analysis by the CCK-8 Kit of MS1 cells that were cultured at 16 mM glucose and treated with PBS (Vehicle, Veh.) or 1 μg/mL recombinant human TSP1 protein in the absence or presence of 1 μg/mL anti-CD47 neutralizing antibody for 48 hours ( n = 6 independent treatment experiments). Data are shown as mean ± SEM by two-way ANOVA ( a – d , g , i , * indicates shCtrl versus shErn1 ; # indicates shErn1 versus shErn1/shThbs1 in i ) or unpaired two-tailed Student’s t -test ( e , f , j ).

Article Snippet: Cells were treated with recombinant human TSP1 protein (ABclonal, RP01197) in the absence or presence of anti-CD47 neutralizing antibody (Bio X Cell- InVivo MAb, BE0283; Clone MIAP410) or IgG control (Bio X Cell- InVivo MAb, BE0083; Clone MOPC-21).

Techniques: Infection, shRNA, Control, Quantitative RT-PCR, Cell Culture, Western Blot, Cell Characterization, CCK-8 Assay, Recombinant, Two Tailed Test